An energy-conserving pyruvate-to-acetate pathway in Entamoeba histolytica. Pyruvate synthase and a new acetate thiokinase.
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An energy-conserving pyruvate-to-acetate pathway in Entamoeba histolytica. Pyruvate synthase and a new acetate thiokinase.
Under anaerobic conditions, cells of Entamoeba histolytica grown with bacteria produce H2 and acetate while cells grown axenically produce neither. Aerobically, acetate is produced and O2 is consumed by amebae from either type of cells. Centrifuged extracts, 2.4 x 106 x g x min, from both types of cells contain pyruvate synthase (EC 1.2.7.1) and an acetate thiokinase which, together, form a sys...
متن کاملAn Energy-conserving Pyruvate-to-Acetate Pathway in Entamoeba histolytica
tica grown with bacteria produce H, and acetate while cells grown axenically produce neither. Aerobically, acetate is produced and 0, is consumed by amehae from either type of cells. Centrifuged extracts, 2.4 x 10” x g x min, from both types of cells contain pyruvate synthase (EC 1.2.7.1) and an acetate thiokinase which, together, form a system capable of converting pyruvate to acetate. Pyruvat...
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Studies in this laboratory have aimed at a comparison of pyruvate and acetate as carbon sources for amino acids in bakers’ yeast (l-3). Aspartic acid in particular was shown (3) to arise from pyruvate largely through a C&-C1 condensation, and from acetate perhaps via a direct coupling of Cs units. In the present paper, these studies have been extended to include the formation of glutamic acid. ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1977
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)32778-3